This study was carried on 89 vancomycin resistant staphylococci (VRS) that were collected from different clinical samples including urine, sputum, nasal swaps and wound pus from Tanta university hospital. Out of these, 88 (98.8 %) were vancomycin resistant Staphylococcus aureus (VRSA) and only one vancomycin resistant caogulase negative staphylococci (1.2 %). The highest percentage of VRS was isolated from wound pus followed by sputum while the least percentage was from nasal swaps and urine. MICs of vancomycin against VRS isolates were determined by agar dilution method following CLSI guidelines. Biofilm formation was tested by adherence of selected isolates on the wells of microtitration plates using crystal violet. It was found that biofilm formation increased in highly resistant VRS isolates. Cell surface hydrophobicity of the selected isolates was measured. The hydrophobicity index (HI) was calculated and it was directly proportional to the vancomycin MICs.
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